The creation of cost-effective cell factories for highly efficient production of proteinases is an urgent task for modern biotechnology. In this work, the bacilisin and bacteriocin genes in the genome of the Bacillus pumilus strain 3-19 were inactivated using the CRISPR-Cas9 system. The growth dynamics and proteolytic activity of the resulting mutant strains were studied.
Bacillus pumilus, proteinases, CRISPR-Cas9, gene inactivation, antimicrobial peptides.
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